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金花茶种子诱导人子宫颈癌HelaS3细胞凋亡及其作用机理的研究(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2013年01期
页码:
112
栏目:
植物化学与化学生理学
出版日期:
2013-01-25

文章信息/Info

Title:
Potential mechanism of Camellia chrysantha seedsinduced apoptosis in human cervical cancer HelaS3 cells
作者:
时贞颂 于大永 彭倩 史丽颖* 冯宝民 王永奇
大连大学 药物研究所,大连 116622
Author(s):
SHI ZhenSong YU DaYong PENG Qian SHI LiYing*FENG BaoMin WANG YongQi
Institute of Materia Medica, Dalian University, Dalian 116622, China
关键词:
-
Keywords:
Camellia chrysantha human cervical cancer HelaS3 cells apoptosis
分类号:
R285.5
DOI:
10.3969/j.issn.1000-3142.2013.01.020
文献标识码:
A
摘要:
为探讨金花茶种子诱导人子宫颈癌HelaS3细胞凋亡的机制,以不同浓度金花茶种子乙醇提取物的正丁醇萃取物处理人子宫颈癌HelaS3细胞后,分别采用流式细胞仪、Western印记法等检测对细胞凋亡及Bax、Bcl2、Bid等表达的影响。结果表明:金花茶种子乙醇提取物的正丁醇萃取物作用于人子宫颈癌HelaS3细胞后,细胞凋亡增加,Bax蛋白表达提高,Bcl2蛋白表达降低,Bid蛋白活性减弱,线粒体膜电位下降及Caspase8,Caspase3活性增强。金花茶种子乙醇提取物的正丁醇萃取物为其重要的抗肿瘤活性有效部位,并可能通过激活Bax及Caspase3,Caspase8,抑制Bcl2和Bid来诱导HelaS3细胞凋亡。
Abstract:
To investigate the effect of the seeds of Camellia chrysantha on apoptosis in human cervical cancer HelaS3 cells. Human HelaS3 cells were treated with the solution of BuOH fraction from the EtOH extract from the seeds of C.chrysantha at different concentrations. The apoptosis was detected by flow cytometry (FCM),apoptosis associated proteins were determined by western blot. After treatment with the solution of BuOH fraction from the EtOH extract of the seeds of C.chrysantha on human HelaS3 cells,a significant enhancement of apoptosis was observed,the expression of Bax was enhanced,the expression of Bcl2 and Bid decreased,and the decrease of mitochondrial membrane potential (MMP) and the activation of Caspase8 and Caspase3 were observed. The BuOH fraction from the EtOH extract of the seeds of C.chrysantha was the active anticancer fraction,which promoted human HelaS3 cells apoptosis possibly by activating Bax,MMP,Caspase8 and Caspase3,and inhibiting Bcl2 and Bid.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2013-01-25