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小桐子果壳杀虫活性部位的分离及其HPLCMS分析(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2013年01期
页码:
107
栏目:
植物化学与化学生理学
出版日期:
2013-01-25

文章信息/Info

Title:
Isolation and HPLCMS analysis insecticidal activity franctions from Japropha curcas fruit shell
作者:
李育川12 郭巧生2* 房海灵23 李维莉1 耿开友1 李绍萍1 王崇亮1
1.昆明学院, 昆明 650214; 2.南京农业大学 中药材研究所, 南京 210095; 3.江西省林科院, 南昌 330032
Author(s):
LI YuChuan 12 GUO QiaoSheng2* FANG HaiLing23LI WeiLi1 GENG KaiYou1LI ShaoPing1 WANG ChongLiang1
1.Kunming University, Kunming 650214, China; 2.Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Najing 210095, China; 3.Jiangxi Academy of Forest, Nanchang 330032, China
关键词:
小桐子果壳 杀虫活性物质 分离鉴定 HPLCMS
Keywords:
Jatropha curcas fruit shell insecticidal activity components isolation and identification HPLCMS
分类号:
Q946
DOI:
10.3969/j.issn.1000-3142.2013.01.019
文献标识码:
A
摘要:
为寻找小桐子果壳的杀虫活性物质,以小桐子果壳乙醇粗提物的正丁醇萃取相为实验材料,对其采用硅胶柱反复柱层析,同时对各阶段分离产物进行活性追踪,筛选出具有杀虫作用的活性部位WD1,最后采用HPLCMS分析,初步鉴定出活性部位主体成分。结果表明,WD1活性组分在浓度为8 g?L1时,对桃蚜(Myzus persicae)和菜青虫(Pieris rapae)的24 h(72 h)校正死亡率均大于70%,显示出极高的毒杀活性。通过对WD1进行HPLCMS分析,鉴定了含量较高的7个化合物,为总量的80.72%,初步鉴定为2a,3a,24三羟基1220(30)〖JP2〗二烯28乌索酸、环辛肽B、牡荆素、松香烷二萜、麻疯树酚酮A、Curcusone A或Curcusone B。
Abstract:
To seek insecticidal activity components from Jatropha curcas fruit shell, using alcohol crude extract of butanol partitioned extract in J.curcas fruit shells as an experimental material in our investigation,and then silica gel chromatography,at same time, separated products of the each stage were carried out by tracking activity,analyzed by HPLCMS,and the main components of active part were initially identified. The results showed that the concentration of colony WD1 reached 8 g?L1,Myzus persicae and Pieris rapae corrected mortality exceeded 70% in 24 h(72 h),which showed that toxic activity was very high. Through the analysis of WD1 by HPLCMS,seven compounds with maximum contents were identified,with total amount up to 80.72%. They were 2α,3a,23Trihydroxyurs12,20(30)dien28oi,Cyclgrossine B,Vitexin,Diterpene ,Jatrophlone A,Curcusone A or Curcusone B.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2013-01-25