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中国石竹离体快繁与试管苗玻璃化研究(PDF)

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2012年04期
页码:
531
栏目:
植物生理与分子生物学
出版日期:
2012-07-25

文章信息/Info

Title:
Study on in vitro propagation and tube plant vitrification of Dianthus chinensis
作者:
程云清1 刘剑锋1 刘春明1 邹振峰2 王淑范3
1.吉林师范大学 生命科学学院, 吉林 四平 136000; 2.四平市林业科学研究院, 吉林 四平 136000; 3.四平市园林管理局, 吉林 四平 136000
Author(s):
CHENG YunQing1 LIU JianFeng1 LIU ChunMing1 ZOU ZhenFeng2 WANG ShuFan3
1.College of Life Sciences, Jilin Normal University, Siping 136000, China; 2.Siping Academy of Forestry Science, Siping 136000, China; 3.Garden Bureau of Siping City, Siping 136000, China
关键词:
中国石竹 组培快繁 玻璃化
Keywords:
Dianthus chinensis tissue culture and rapid propagation vitrification
分类号:
S792.11
DOI:
-
文献标识码:
A
摘要:
研究了培养基组成成分对中国石竹品种Diana F1White组培程序中种子萌发、诱芽增殖、试管苗玻璃化及生根等重要环节的影响。结果表明:(1)种子适宜的萌发培养基为1/2 MS,在此培养基中种子萌发率为83.33%,播种后30 d时无菌苗高度达6.99 cm,叶片数达26.7。(2)在芽诱导阶段玻璃化苗发生率最高可达53.85%。将光照强度提高至2 000 lx后,采用培养基MS+6BA 3.0 mg/L+NAA 0.3 mg/L+琼脂8.0 g/L+蔗糖8.0 g/L进行诱芽培养,玻璃化苗发生率降至3.33%,外植体出芽率达到90%,单个外植体出芽数达到7.2个。(3)适宜的生根培养基为1/2 MS+1.0 mg/L IBA+琼脂8 g/L+蔗糖40 g/L,培养30 d时生根率为100%,平均根条数达到24.7条,平均根长度达到4.7 cm。试管苗在消毒后的腐殖土中移栽,成活率达95%。该研究结果为Diana F1试管苗的快速繁殖提供科学依据。
Abstract:
Effects of medium ingredients on seeds germination,buds inducement proliferation,tube plant vitrification and rooting were studied in order to establish efficient rapid propagation technology system of Dianthus chinensis cultivar Diana F1 White. The results showed that proper seeds germination medium was 1/2 MS,and seed germination ratio was 83.33%. Height of aseptic tube plant reached to 6.99 cm,and leaf number per plant was 26.7 on 30th day after sowing in medium. Max.vitrification plant ratio was 83.33% during bud inducement period for tube plant of Diana F1 White. The optimal medium for buds inducement was MS+6BA 3.0 mg/L+NAA 0.3 mg/L+agar 8.0 g/L+sugar 8.0 g/L after light intensity was promoted to 2 000 lx. Bud inducement ratio and buds number per explant were 90% and 7.2 respectively,and the ratio of vitrification tube plants decreased to 3.33% after 30 days’culture. The optimal rooting medium was 1/2 MS+1.0 mg/L IBA+agar 8 g/L+sugar 40 g/L,and rooting percent,roots number and average root length was 100%,24.7 and 4.7 cm respectively after 30 days’ culture. Survival rate of tube plant was more than 95% after they were transplanted in disinfected humus. The above results would provide scientific base for micro propagation of D.chinensis cultivar Diana F1 White.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2012-07-25