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|本期目录/Table of Contents|

中国板栗EST-SSR分子标记在栲树中的通用性分析

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2012年03期
页码:
293-297
栏目:
系统演化植物学与生物地理学
出版日期:
2012-07-05

文章信息/Info

Title:
Transferability analysis of ESTSSR markers of Castanea mollissima to Castanopsis fargesii
作者:
李 春 孙 晔
1.中国科学院 华南植物园, 广州 510650; 2.中国科学院 研究生院, 北京 100049; 3.中国科学院 华南植物园 植物资源保护与可持续利用重点实验室, 广州 510650
Author(s):
LI Chun12 SUN Ye13*
1.South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; 2.Graduate School of Chinese Academy of Sciences, Beijing 100049, China; 3.Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
关键词:
中国板栗 栲树 ESTSSR 通用性
Keywords:
Castanea mollissima Castanopsis fargesii ESTSSR transferability
分类号:
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DOI:
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文献标识码:
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摘要:
简单重复序列也称为微卫星分子标记,不仅在同属近缘种间具有良好的通用性,甚至在近缘属间也具有一定的通用性。本研究利用壳斗科基因组信息数据库中公布的中国板栗124对多态的ESTSSR引物在栲树中进行跨属(栗属到栲属)通用性研究,结果显示中国板栗ESTSSR引物在栲树中通用性和多态性分别为42.7%和56.6%;使用19对多态的ESTSSR引物对4个栲树自然居群的遗传多样性进行初步分析,结果显示栲树自然居群具有较高的遗传多样性(Na=6.105,Ho=0.563,He=0.621)。这些引物为栲树群体遗传学的深入研究提供了有力工具。
Abstract:
Simple sequence repeats(SSR),also known as microsatellite molecular markers,have been crossamplified successfully in closely related species of the same genus and even across genera within the same family. In the present study,124 primer pairs of polymorphic ESTSSR originally developed from Castanea mollissima were crossamplified in Castanopsis fargesii. Results indicated that 42.7% of Castanea mollssima ESTSSR primers were successfully crossamplified in Castanopsis fargesii and 56.6% were polymorphic. The genetic diversity of 4 populations of C.fargesii were investigated with polymorphic ESTSSRs,preliminary results showed that C.fargesii possessed high levels of genetic diversity(Na=6.105,Ho=0.563,He=0.621). These polymorphic ESTSSR primers would provide a powerful tool for further investigation on population genetics of C.fargesii.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2012-05-25