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胶束电动毛细管色谱法测定红丝线提取物中紫蓝素的含量

《广西植物》[ISSN:1000-3142/CN:45-1134/Q]

期数:
2010年05期
页码:
707-709
栏目:
植物生物化学与化学生物学
出版日期:
2010-09-15

文章信息/Info

Title:
Determination of zilanine contents in extracts of Peristrophe baphica by Micellar Eletrokinetic Capillary Chromatography (MECC)
作者:
李 娟 谢运昌 蒋小华
广西壮族自治区中国科学院广西植物研究所, 广西 桂林 541006
Author(s):
LI Juan XIE YunChang* JIANG XiaoHua
Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China
关键词:
胶束电动毛细管电泳色谱法 紫蓝素 红丝线提取物
Keywords:
MECC Zilanine extract of Peristrophe baphica
分类号:
-
DOI:
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文献标识码:
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摘要:
建立了胶束电动毛细管电泳色谱法(MECC)测定红丝线提取物中紫蓝素的含量的方法。毛细管柱内径75 μm,长50.2 cm;运行电压25 kV,检测波长585 nm,温度25 ℃;缓冲液为25 mmol/L,βCD 10 mmol/L,硼酸盐20%乙腈(pH值8.0);进样方式:压力进样,进样时间5 s。结果表明紫蓝素在10~100 μmol/L浓度范围内具有良好的线性关系(r=0.9995),回收率为95.3%~103.2%。该方法快速、简便、并且较为准确,适用于测定红丝线提取物中紫蓝素的含量。
Abstract:
The method of MECC was established to determine zilanine contents in extracts of P.baphica. The capillary column was 50.2 cm long and 75 μm in internal diameter;the operation voltage was 25 kV,testwavelength 585 nm,column temperature 25 ℃;the mobile buffer was 25 mmol/L boric acid20% acetonitrile(pH 8.0)with 10 mmol/L βCD;the sampling was conducted by pressure for 5 s. The results showed that the linear range of determination for zilanine was 10~100 μmol/L(r=0.9995). The average recovery rate was 953%-103.2%. The method was fast,simple,convenient,comparatively accurate,and could be used to determinate zilanine contents in extract of P.baphica.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2010-09-01